Flow Cytometry Specialist I/II
The La Jolla Institute for Immunology is dedicated to understanding the intricacies and power of the immune system. Unraveling and recognizing mechanisms is key to be able to apply that knowledge and thereby promote human health and prevent immune system related diseases. Research at the La Jolla Institute is focused on a wide range of immune disorders and diseases, including infectious diseases such as COVID-19, ZIKA, Dengue, tuberculosis and HIV, autoimmune diseases such as inflammatory bowel disease, diabetes, and allergies and cancer immunotherapy.
One of the most valuable tools in immunology research is flow cytometry. This high throughput technology is used to analyze the physical and chemical characteristics of particles – mostly cells - in a stream of fluid as it passes through laser light. Cell components are fluorescently labeled and then excited by the laser to emit light at varying wavelengths. In immunology research and healthcare, flow cytometry has numerous essential applications including cell counting, cell sorting, detection of biomarkers, phenotyping of disease specific cell populations, and clinical diagnostics, etc. With the help of this technology, data of a large number of cells can be ascertained over a short period. Information on the heterogeneity and different subsets within cell populations can be identified and measured and specific signatures deciphered.
The Flow Cytometry Core Facility at the La Jolla Institute for Immunology is one of the largest shared resource laboratories at a non-profit institution in San Diego. The LJI Flow Cytometry Core Facility maintains and supports a total of 17 instruments, including novel high parameter technology such as the Cytek Aurora CS, BD FACSymphony S6 and a Fluidigm CyTOF Helios mass cytometer (cytometry by time-of-flight mass spectrometry), with plans to expand and purchase more instrumentation. The LJI Flow Core assists over 400 scientists and researchers at LJI, and provides services to UCSD and external collaborators. Our overall goal in the Flow Core is to provide cutting-edge technology and resources along with the expertise to guide and assist new and existing users of our facility (in cytometry related projects).
Essential Duties and Responsibilities
- Perform daily startup, calibration, operation and shutdown of all sorters and analyzers
- Routine maintenance - including decontamination of analyzers and sorters
- Preparing sorters for aseptic sorts
- Reviewing and answering scheduling requests for sorters and analyzers
- Supervised troubleshooting of all sorters and analyzers
- Assist users in experiment setup and instrument troubleshooting for both sorters and analyzers
- Monitoring sorts (changing collection tubes, adjusting flow rates, changing inline filters in case of clogging)
- Restocking consumables and keeping the laboratory spaces (sort and analyzer room) clean and organized
- Bachelor’s or Master’s in biological sciences or engineering preferred
- Benchwork experience a plus
- Must be able to work flexible hours
With over 20 different countries represented, diversity is respected and celebrated at LJI. We are committed to ensuring a safe and compassionate environment that is equal, just, and fair. Science and discovery benefit from a diversity of talents, a diversity of approaches, and a diversity of perspectives. The La Jolla Institute’s Diversity, Equity, and Inclusion Initiative is designed to help us identify areas at the Institute that need improvement so we can together create an environment that is inclusive, provides equal opportunities, and opens doors that propel our research for human health.
- Job Function RA
- Pay Type Salary
- 9420 Athena Cir, La Jolla, CA 92037, USA