Senior Flow Cytometry Specialist
The La Jolla Institute for Immunology is dedicated to understanding the intricacies and power of the immune system. Unraveling and recognizing mechanisms is key to be able to apply that knowledge and thereby promote human health and prevent immune system related diseases. Research at the La Jolla Institute is focused on a wide range of immune disorders and diseases, including infectious diseases such as COVID-19, ZIKA, Dengue, tuberculosis and HIV, autoimmune diseases such as inflammatory bowel disease, diabetes, and allergies and cancer immunotherapy.
One of the most valuable tools in immunology research is flow cytometry. This high throughput technology is used to analyze the physical and chemical characteristics of particles – mostly cells - in a stream of fluid as it passes through laser light. Cell components are fluorescently labeled and then excited by the laser to emit light at varying wavelengths. In immunology research and healthcare, flow cytometry has numerous essential applications including cell counting, cell sorting, detection of biomarkers, phenotyping of disease specific cell populations, and clinical diagnostics, etc. With the help of this technology, data of a large number of cells can be ascertained over a short period. Information on the heterogeneity and different subsets within cell populations can be identified and measured and specific signatures deciphered.
The Flow Cytometry Core Facility at the La Jolla Institute for Immunology is one of the largest shared resource laboratories at a non-profit institution in San Diego. The LJI Flow Cytometry Core Facility maintains and supports a total of 17 instruments, including novel high parameter technology such as the Cytek Aurora CS, BD FACSymphony S6 and a Fluidigm CyTOF Helios mass cytometer (cytometry by time-of-flight mass spectrometry), with plans to expand and purchase more instrumentation. The LJI Flow Core assists over 400 scientists and researchers at LJI, and provides services to UCSD and external collaborators. Our overall goal in the Flow Core is to provide cutting-edge technology and resources along with the expertise to guide and assist new and existing users of our facility (in cytometry related projects).
Essential Duties and Responsibilities
- Training and advising an interdisciplinary team of principal investigators, postdoctoral scientists, and research technicians with both flow and mass cytometry experiments
- Training and mentoring other core staff members
- Perform daily calibration, operation, shutdown, routine maintenance, troubleshooting and repair of all sorters and analyzers and the CyTOF
- Coordinating instrument repairs with service engineers
- Provide training and assistance to users with multi-parameter panel design, instrument setup and data acquisition, as well as special applications on different types of cytometers
- Staff-operated cell sorting services (performance of low, medium, and high-speed bulk and custom multi-parameter cell sorting of various cell lines and cell types from murine and human tissue/organs)
- New instrument evaluation and validation of hardware and software, including implementation of novel applications on the sorters (high-parameter panels, 6-way sorts, rare population sorts into specific collection devices, single-cell sorts etc.)
- Help with data analysis and advanced computational tools using various computing platforms including FACS Diva, FACS Chorus, FlowJo, Cytobank and OMIQ
- Assist facility users with troubleshooting during acquisition and data analysis
- Assistance with preparation, review and submission of instrumentation grants and manuscripts
- Attend national and international scientific research and/or administrative conferences or training workshops, internal LJI seminar series and technology-oriented meetings to stay up to date with cutting edge technology in the field
- Bachelor’s or Master’s degree, or PhD (preferred) in Biological Sciences, Engineering or related field with 6-8 years or more experience in the field of flow cytometry
- Previous experience working in a flow cytometry core facilitysetting with multiple platforms
- Previous lab experience performing high parameter immunophenotyping
- Must be able to work flexible hours
With over 20 different countries represented, diversity is respected and celebrated at LJI. We are committed to ensuring a safe and compassionate environment that is equal, just, and fair. Science and discovery benefit from a diversity of talents, a diversity of approaches, and a diversity of perspectives. The La Jolla Institute’s Diversity, Equity, and Inclusion Initiative is designed to help us identify areas at the Institute that need improvement so we can together create an environment that is inclusive, provides equal opportunities, and opens doors that propel our research for human health.
- Job Function RA
- Pay Type Salary
- 9420 Athena Cir, La Jolla, CA 92037, USA