Research Tech II (Ollmann-Saphire Lab)

9420 Athena Cir, La Jolla, CA 92037, USA Req #243
Thursday, January 6, 2022

Job Summary

We are looking for two highly motivated Research Technicians with a protein chemistry and characterization background to join the Ollmann-Saphire lab. The successful candidates will support protein cloning, expression, purification, and analytical characterization for vaccine and antibody discovery. These Research Technician positions are a great opportunity for experienced technicians as well as recent graduates to expand their skills in state-of-the-art protein chemistry techniques, vaccinology, and cryo-EM, while accelerating therapeutic discovery and development in translational medicine and structural biology for several viral diseases.

Essential Duties & Responsibilities

      Express and purify recombinant proteins from mammalian cell culture and bacteria

      Characterize purified protein samples using SDS-PAGE, HPLC and other analytical assays

      Isolate RNA from single cells and perform downstream processing (reverse transcription and PCR) to obtain paired antibody sequences

      Maintain a detailed and well-organized record of experiments in a notebook

      Work independently while adhering to established protocols

      Organize time and plan specifics of project related work with oversight from manager

      Troubleshoot technical and instrumentation issues and recommend solutions

 

Key skills preferred:

      Experience with mammalian cell culture

      Hands-on experience with recombinant protein purification utilizing AKTA system

      Expertise in analytical characterization methods such as SDS-PAGE, HPLC and SEC-MALS

      Experience performing cell-based assays to identify functional antibody candidates and/or biochemical characterization (ELISA, BLI, SPR) to determine binding specificities and binding kinetics

      Experience with multi-color flow cytometry

      Experience with NGS and/or bioinformatics

      Ability to work in fast paced environments and make contributions to multiple projects

      Attention to detail, self-motivation, and creative thinking will be essential

 

Leveling Requirements

      Requires a BA/BS Degree with two years prior laboratory experience, or an MS Degree with demonstrated working knowledge of scientific principles.

      Clear and effective written and verbal communication skills

      Excellent organizational skills with strong attention to detail and ability to multi-task

      Familiarity with and enthusiasm for basic biological research

      Pertinent laboratory experience will be favorably considered


LJI provides Equal Employment Opportunity (EEO) to all employees and applicants regardless of race, national origin, religion, sex, marital status, age, mental or physical disability, medical condition, veteran status, sexual orientation, or pregnancy. This applies to all personnel practices, including recruitment, hiring, training, promotion, compensation, benefits, transfers, educational assistance, and social and recreational programs.


With over 20 different countries represented, diversity is respected and celebrated at LJI. We are committed to ensuring a safe and compassionate environment that is equal, just, and fair. Science and discovery benefit from a diversity of talents, a diversity of approaches, and a diversity of perspectives. The La Jolla Institute’s Diversity, Equity, and Inclusion Initiative is designed to help us identify areas at the Institute that need improvement so we can together create an environment that is inclusive, provides equal opportunities, and opens doors that propel our research for human health. 

Other details

  • Job Function RT
  • Pay Type Salary
Location on Google Maps
  • 9420 Athena Cir, La Jolla, CA 92037, USA